Platelet and coagulation function before and after burn and smoke inhalation injury in sheep.

Background: Smoke inhalation and burn injury remain a major source of morbidity and mortality. There is known dysregulation of hemostasis in burn patients, but either hyper- or hypocoagulation states are reported. Sheep are an established animal model for studying burn pathology and provide robust data on hemostatic function at baseline and after injury. Methods: Following an IACUC-approved protocol, 15 sheep were anesthetized and subjected to a 40% full thickness burn with smoke inhalation. Blood was sampled at baseline, 1 day post injury (early effects) and days 2, 3 and 4 (late effects) after injury. Assays at each time point assessed: hemostatic function by thromboelastography; platelet counts and function by flow cytometry and aggregometry; coagulation protein levels; and free hemoglobin. Data was analyzed by the Wilcoxon paired test (non-parametric) with significance set at

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