This article presents a basic scheme for a multichannel combined optoelectrode microimplant using feedback developed by the authors. An algorithm for stimulation and recording of neuron responses able to adjust the command signals to the light source and an optional system for administration of pharmacological compounds are described. The device can be used for autonomous adaptive optogenetic stimulation in chronic experiments on freely mobile animals, and has potential for use in treating patients. This report provides a detailed description of the preparation of a combined optoelectrode microimplant (optrode). The main advantage of this development is the ability to combine the stimulating and recording parts of the optogenetic system with adaptive control of light source parameters without the system being permanently connected to a personal computer. The system will later be tested on slices and in freely mobile animals in studies of the electrophysiological characteristics of hippocampal neurons in transgenic mice with different models of neurodegenerative diseases.
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