Evaluation of adenosine, lidocaine and magnesium for enhancement of platelet function during storage.

Background: The combination of Adenosine, lidocaine and Mg2+ (ALM) has demonstrated cardioprotective and resuscitative properties in models of cardiac arrest and hemorrhagic shock that are linked to reduction of metabolic demand. Platelets play a key role in resuscitation strategies for ATC, but suffer from loss of function following storage in part due to mitochondrial exhaustion. This study evaluates whether ALM also demonstrates protective properties in stored platelet preparations. Methods: Platelets were tested at (Baseline (BL), Day 5, 10, and 15) at 22[degrees]C (RT) or 4[degrees]C in 100% plasma and platelet additive solution (PAS). ALM treatment or its individual components (A, L, M or combinations) were added directly to the minibags at BL for storage. Measurements consisted of blood gas and chemisty analyses, thromboelastography (TEG), impedance aggregometry, and flow cytometry. Results: Blood gas and cell analysis, as well as flow cytometry measures, demonstrated only differences between temperature groups starting at Day 5 (P

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