Biliary tract external drainage improves inflammatory mediators and pathomorphology of the intestine, liver, and lung in septic rats

Background To investigate the effect of biliary tract external drainage (BTED) on inflammatory mediators and pathomorphism of intestine, liver, and lung in septic rats. Method 48 SD rats (n=8 per group) were randomized into six groups: control, sepsis, sepsis plus BTED, normal bile (obtained from 8 healthy rats) and septic bile infusion for 6 hours respectively to test the effects of BTED bile infusion on cytokines’ expression and tissue injury in the intestine, liver, and lung of septic/normal rats. Co-cultivation of intestinal epithelial cells (IEC-6) with bile for 12 hours was performed to evaluate the potential cytotoxicity of septic bile. Survival rate for sepsis plus BTED rats was detected compared with sepsis without BTED group (n=20 per group) at 24, 48 and 72 hours respectively. Results BTED for 6 hours significantly reduced the mRNA expression levels of TNF-α and IL-1β (all P

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