Ethylene Response Factor (ERF) Family Proteins in Abiotic Stresses and CRISPR–Cas9 Genome Editing of ERFs for Multiple Abiotic Stress Tolerance in Crop Plants: A Review

Abstract

Abiotic stresses such as extreme heat, cold, drought, and salt have brought alteration in plant growth and development, threatening crop yield and quality leading to global food insecurity. Many factors plays crucial role in regulating various plant growth and developmental processes during abiotic stresses. Ethylene response factors (ERFs) are AP2/ERF superfamily proteins belonging to the largest family of transcription factors known to participate during multiple abiotic stress tolerance such as salt, drought, heat, and cold with well-conserved DNA-binding domain. Several extensive studies were conducted on many ERF family proteins in plant species through over-expression and transgenics. However, studies on ERF family proteins with negative regulatory functions are very few. In this review article, we have summarized the mechanism and role of recently studied AP2/ERF-type transcription factors in different abiotic stress responses. We have comprehensively discussed the application of advanced ground-breaking genome engineering tool, CRISPR/Cas9, to edit specific ERFs. We have also highlighted our on-going and published R&D efforts on multiplex CRISPR/Cas9 genome editing of negative regulatory genes for multiple abiotic stress responses in plant and crop models. The overall aim of this review is to highlight the importance of CRISPR/Cas9 and ERFs in developing sustainable multiple abiotic stress tolerance in crop plants.

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Detection of Emotions Induced by Colors in Compare of Two Nonlinear Mapping of Heart Rate Variability Signal: Triangle and Parabolic Phase Space (TPSM, PPSM)

Abstract

Purpose

Emotions in the word mean understanding, sensing and recognizing something with one's senses. Emotions are relatively simple responses that the body presents to external or internal stimuli. As the individual's mental and emotional state changes, the activity of the various parts of the body also changes and increases. This applies to all cultures. So emotion detection from biological signals especially heart rate variability (HRV) signal is considered in this article.

Methods

The behavior of HRV in two nonlinear phase space have been evaluated and the extracted features of these two mapping have been used for emotion detection. Triangle and Parabolic Phase Space Mapping (TPSM and PPSM) are recently introduced methods which are able to indicate hidden aspects of HRV in response to different emotions. The Lead II of electrocardiogram signal was recorded from 32 female students while they were stimulated by four main psychology colors (blue, green, red, and yellow) to induce emotions which were labeled by SAM test.

Results

The results show that the mentioned colors caused emotions pleasure, joy, anger, and sadness, respectively. After rating the results by Kruskal–Wallis test, k-nearest neighbor classifier was used for emotion classification. The obtained accuracy after 10-fold cross validation was 90.91%.

Conclusions

The hypotheses of being a relationship between colors and emotions without awareness of subjects have been proved. Furthermore, by studying the usefulness of TPSM and PPSM as new mapping of HRV, significant results have obtained which indicate the advantages of using them. So it is suggested to use and analyze the usage of these kinds of colors in different psychological situations and for biofeedback tests.

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Beliefs and Attitudes About the Dissemination and Implementation of Internet-Based Self-Care Programs in a Large Integrated Healthcare System

Abstract

Behavioral intervention technologies (BITs) are online programs or mobile applications that deliver behavioral health interventions for self-care. The dissemination and implementation of such programs in U.S. healthcare systems has not been widely undertaken. To better understand these phenomena, we explored perspectives on BIT deployment in the Veterans Health Administration. Interviews from 20 providers, administrators, and policy makers were analyzed using qualitative methods. Eight themes were identified including the use of traditional healthcare delivery models, strategies for technology dissemination and implementation, internet infrastructure, leadership, health system structure, regulations, and strategic priorities. This research suggests policy, funding, and strategy development initiatives to promote the implementation and dissemination of BITs.

http://bit.ly/2F4NBap

Associations of Socio-demographic, Family, and Neighborhood Factors with Physical Activity-Related Parenting Practices Among Hong Kong Preschoolers’ Parents

Abstract

Objectives Regular engagement in physical activity (PA) has numerous health benefits in young children. Young children's parents can influence their children's PA behavior through different PA-related parenting practices. This cross-sectional study examined the independent contributions of socio-demographic, family/home and parent-perceived neighborhood environmental characteristics explaining PA-related parenting practices encouraging or discouraging PA among Hong Kong preschool-aged children (3–5 years-old). Methods Hong Kong Chinese preschoolers' parents were recruited from pre-selected kindergartens and Maternal and Child Health Centers located in areas stratified by residential density and socio-economic status. They self-completed socio-demographic, family/home and perceived neighborhood characteristics and PA-related parenting practices questionnaires. Generalized linear models were used to examine associations of socio-demographic, family/home and neighborhood variables with PA-related parenting practices. Results Socio-demographic and family/home characteristics were significantly correlated with parenting practices encouraging and discouraging PA. Parent-perceived neighborhood characteristics were significantly correlated with parenting practices discouraging PA only. Conclusions for Practice This study identified correlates of PA-related parenting practices among parents of Hong Kong Chinese preschoolers. The findings suggest future PA-promoting interventions among Chinese preschoolers via the promotion of parenting practices encouraging children's PA should consider multiple factors, including family relationships and childcare sharing, promotion of PA and its benefits among parents, and neighborhood social cohesion, traffic safety and safety from crime.

http://bit.ly/2Qhn5w6

Socio-Structural Factors Influencing the Prevention of Mother-to-Child Transmission of HIV in the Democratic Republic of the Congo: A Systematic Review

Abstract

Introduction The Democratic Republic of the Congo (DRC) had the second-highest mother-to-child HIV transmission rate in Sub-Saharan Africa at 29% in 2013, however the uptake of preventive services for mother-to-child transmission of HIV (PMTCT) remains suboptimal. This systematic review explores socio-cultural and structural factors influencing PMTCT service uptake in the DRC. Methods We conducted a search of electronic databases. The PEN-3 model was used as a framework to synthesize factors influencing PMTCT service uptake into perceptions, enablers, and nurturers. Results Sixteen studies, 14 quantitative and two qualitative, were retained. Maternal, socio-economic, structural, and cultural factors were found to influence PMTCT service uptake in the DRC. Cost, accessibility, and quality of PMTCT services were key barriers to service uptake. Integration, male partner involvement, and evolving PMTCT policies improved PMTCT service delivery and uptake. This review also revealed several socio-cultural challenges in involving male partners in PMTCT programs. Discussion Findings highlight the need to address barriers and reinforce enablers of PMTCT uptake. Creating culturally appropriate, male-friendly, and family-oriented PMTCT programs will improve service uptake in the DRC.

http://bit.ly/2Vp4pOL

First-Generation Immigrant Mothers Report Less Spanking of 1-Year-Old Children Compared with Mothers of Other Immigrant Generations

Abstract

Introduction The American Academy of Pediatrics discourages spanking, especially of infants and young toddlers. This study examines the association between maternal immigrant generation and reported spanking of 1-year-old children, and whether this association is impacted by domestic violence (DV). Methods We conducted a cross-sectional secondary data analysis using 1-year wave data from the Fragile Families and Child Wellbeing Study. We used descriptive statistics to explore demographic differences among first-generation, second-generation, and third-generation or higher (reference group) mothers. We conducted logistic regression to examine the association between immigrant generation and spanking, controlling for covariates. We used stratified logistic regression to evaluate how experiencing DV may impact the association between immigrant generation and spanking. Results The study included 370 first-generation mothers, 165 second-generation mothers, and 1754 reference group mothers. The prevalence of spanking differed across immigrant generations (p = 0.004). First-generation mothers had statistically significant lower odds of spanking compared with the reference group (adjusted OR 0.26; CI 0.11–0.64). Second-generation mothers also had lower odds of spanking compared with the reference group, although this result did not reach statistical significance (adjusted OR 0.60; CI 0.22–1.63). Mothers' report of experiencing DV appeared to impact the relationship between immigrant generation and spanking. Discussion First-generation immigrant mothers had lower odds of reported spanking compared to reference group mothers, an association which is attenuated for both second-generation immigrant mothers and mothers who have experienced DV. Future work should explore the potential factors that drive variations in spanking between immigrant generations.

http://bit.ly/2QdIYfP

Screening for Pregnancy Status in a Population-Based Sample: Characteristics Associated with Item Nonresponse

Abstract

Objectives

Population-based recruitment of a cohort of women who are currently pregnant or who may become pregnant in a given timeframe presents challenges unique to identifying pregnancy status or the likelihood of future pregnancy. Little is known about the performance of individual eligibility items on pregnancy screeners although they are critical to participant recruitment. This paper examined the patterns and respondent characteristics of key pregnancy screener items used in a large national study.

Methods

Cross-sectional analyses were conducted. Descriptive statistics and multivariable logistic regression models were used to examine nonresponse patterns to three questions (currently pregnant, trying to get pregnant and able to get pregnant). The questions were asked of 50,529 women in 17 locations across the US, as part of eligibility screening for the National Children's Study Vanguard Study household-based recruitment.

Results

Most respondents were willing to provide information about current pregnancy, trying, and able to get pregnant: 99.3% of respondents answered all three questions and 97.4% provided meaningful answers. Nonresponse ranged from 0.3 to 2.5% for individual items. Multivariable logistic regression results identified small but statistically significant differences in nonresponse by respondent age, marital status, race/ethnicity-language, and household-based recruitment group.

Conclusions for Practice

The high levels of response to pregnancy-related items are impressive considering that the eligibility questions were fairly sensitive, were administered at households, and were not part of a respondent-initiated encounter.

http://bit.ly/2VnqRIj